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Broad Institute Inc
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GenScript corporation
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Novoprotein
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Image Search Results
Journal: Poultry Science
Article Title: A RT-ERA-CRISPR/Cas12a assay for rapid point-of-care duck hepatitis A virus detection
doi: 10.1016/j.psj.2025.105316
Figure Lengend Snippet: Establishment of the RT-ERA/LbCas12a fluorescence assay. (A) Optimization of crRNA3 concentration. The crRNA3 was diluted to five different concentrations 12.5 nM, 25 nM, 50 nM, 100 nM, and 200 nM, and added to the reaction system. The reaction mixtures were incubated for 10, 20, 30, 45 or 60 min and then visualized under UV light. (B) Optimization of the LbCas12a concentration. LbCas12a was diluted to five different concentrations, and each concentration was added to the reaction system. The reaction mixtures were incubated and visualized under UV light.
Article Snippet:
Techniques: Fluorescence, Concentration Assay, Incubation
Journal: Poultry Science
Article Title: A RT-ERA-CRISPR/Cas12a assay for rapid point-of-care duck hepatitis A virus detection
doi: 10.1016/j.psj.2025.105316
Figure Lengend Snippet: RT-ERA/LbCas12a-LFA assay establishment. (A) Optimization of the FAM-biotin-conjugated ssDNA probe. (B) Optimization of crRNA3 concentration. (C) Optimization of the LbCas12a concentration. The reaction system of the RT-ERA/LbCas12a-LFA assay, including different crRNA3 concentrations, LbCas12a concentrations and ssDNA reporter concentrations was determined using the cleavage efficiency of the LbCas12a protein.
Article Snippet:
Techniques: Concentration Assay